Author/Authors :
Lagomarsino، نويسنده , , Stefano and Iotti، نويسنده , , Stefano and Farruggia، نويسنده , , Giovanna and Cedola، نويسنده , , Alessia and Trapani، نويسنده , , Valentina and Fratini، نويسنده , , Michela and Bukreeva، نويسنده , , Inna and Notargiacomo، نويسنده , , Andrea and Mastrototaro، نويسنده , , Lucia and Marraccini، نويسنده , , Chiara and Sorrentino، نويسنده , , Andrea and McNulty، نويسنده , , Ian and Vogt، نويسنده , , Stefan and Legnini، نويسنده , , Daniel and Kim، نويسنده , , Sangsoo and Gianoncelli، نويسنده , , Alessandra and Maier، نويسنده , , Jeanette A.M. and Wolf، نويسنده , , Federica I. and Di Francesco، نويسنده ,
Abstract :
We report a novel experimental approach to derive quantitative concentration map of light elements in whole cells by combining two complementary nano-probe methods: X-ray fluorescence microscopy (XRFM) and atomic force microscopy (AFM). The concentration is derived by normalizing point-by-point the elemental (here Mg) spatial distribution obtained by XRFM, by the thickness measured using AFM. The considerable difference between the elemental distribution and the concentration maps indicates that this procedure is essential to obtain reliable information on the role and function of elements in whole cells.
Keywords :
X-ray fluorescence microscopy , Cell element concentration , Synchrotron radiation , atomic force microscopy , Magnesium
