Author/Authors :
Po??zka، نويسنده , , P. and Prochazka، نويسنده , , D. and Pil?t، نويسنده , , Z. and Krajcarov?، نويسنده , , Klaus L. E. Kaiser، نويسنده , , J. and Malina، نويسنده , , R. and Novotn?، نويسنده , , J. and Zem?nek، نويسنده , , P. and Je?ek، نويسنده , , J. and ?er?، نويسنده , , M. and Bernatov?، نويسنده , , S. and Krzy??nek، نويسنده , , V. and Dobransk?، نويسنده , , K. and Novotn?، نويسنده , , K. and Trt?lek، نويسنده , , M. and Samek، نويسنده , , O.، نويسنده ,
Abstract :
We report on the application of laser-induced breakdown spectroscopy (LIBS) to the determination of elements distinctive in terms of their biological significance (such as potassium, magnesium, calcium, and sodium) and to the monitoring of accumulation of potentially toxic heavy metal ions in living microorganisms (algae), in order to trace e.g. the influence of environmental exposure and other cultivation and biological factors having an impact on them. Algae cells were suspended in liquid media or presented in a form of adherent cell mass on a surface (biofilm) and, consequently, characterized using their spectra. In our feasibility study we used three different experimental arrangements employing double-pulse LIBS technique in order to improve on analytical selectivity and sensitivity for potential industrial biotechnology applications, e.g. for monitoring of mass production of commercial biofuels, utilization in the food industry and control of the removal of heavy metal ions from industrial waste waters.
Keywords :
LIBS , Double-pulse , Water-jet , Algal biomass , biotechnology
