Title of article :
Use of short high-performance liquid chromatography columns and tandem-mass spectrometry for the rapid analysis of a prostaglandin analog, fluprostenol, in rat plasma
Author/Authors :
Steven H. and Eichhold، نويسنده , , Thomas H and Kuhlenbeck، نويسنده , , Debbie L and Baker، نويسنده , , Timothy R and Stella، نويسنده , , Mark E and Amburgey، نويسنده , , Jack S and deLong، نويسنده , , Mitchell A and Hartke، نويسنده , , James R and Cruze، نويسنده , , Charles F. and Pierce، نويسنده , , Sherie A and Wehmeyer، نويسنده , , Kenneth R، نويسنده ,
Abstract :
A short reversed-phase HPLC column and a tandem mass spectrometer were used to develop a stable-isotope-dilution assay for the rapid and sensitive analysis of fluprostenol, a prostaglandin analog, in rat plasma. A Waters Symmetry ODS column (2.1×10 mm) afforded rapid isocratic elution of fluprostenol (tR=40 s) but still provided a relatively large k′ value of 4. The use of tandem mass spectrometry allowed the interference-free detection of fluprostenol under the rapid elution conditions, with a limit of quantitation of 25 pg ml−1 fluprostenol, using 0.2 ml plasma sample volumes. The method was linear over three orders of magnitude, yielded accurate and precise results and allowed the pharmacokinetic profile of fluprostenol to be defined following intravenous administration in rats.
