Improvements in DNA sequencing by capillary electrophoresis at elevated temperature using poly(ethylene oxide) as a sieving matrix

DNA sequencing in poly(ethylene oxide) (PEO) matrix by capillary electrophoresis was demonstrated at high temperature. The optimal separation temperature is around 40°C. The effects of polymer concentration and types of buffers on the separation performance were investigated. A new buffer system consisting of Tris–Taps–His–EDTA works well with PEO. High-speed separation and good resolution can be fulfilled by using a single-MW PEO polymer. It offers similar separation performance as before for the small DNA fragments, but better performance for large DNA fragments.