Author/Authors :
Guan، نويسنده , , Fuyu and Uboh، نويسنده , , Cornelius E and Soma، نويسنده , , Lawrence R and Birks، نويسنده , , Eric K and Teleis، نويسنده , , Donna and Rudy، نويسنده , , Jeffrey A and Watson، نويسنده , , Anthony O and Tsang، نويسنده , , Deborah S، نويسنده ,
Abstract :
A reliable and sensitive method for the extraction and quantification of phenytoin (5,5′-diphenylhydantoin), its major metabolite, 5-(p-hydroxyphenyl)-5-phenylhydantoin (p-HPPH) and minor metabolite, 5-(m-hydroxyphenyl)-5-phenylhydantoin (m-HPPH) in horse urine and plasma is described. The method involves the use of solid-phase extraction (SPE), liquid–liquid extraction (LLE), enzyme hydrolysis (EH) and high-performance liquid chromatography (HPLC). The minor metabolite, 5-(m-hydroxyphenyl)-5-phenylhydantoin (m-HPPH) was not present in a reliably quantifiable concentration in all samples. The new method described was successfully applied in the pharmacokinetic studies and elimination profile of phenytoin and p-HPPH following oral or intravenous administration in the horse.
