Author/Authors :
Hernلndez، نويسنده , , Raْl and Chong، نويسنده , , Eric and Morales، نويسنده , , Raْl and Pérez، نويسنده , , Ever and Amador، نويسنده , , Yamel and Zubiaurrez، نويسنده , , Jorge R and Valdés، نويسنده , , Rodolfo and Figueroa، نويسنده , , Alejandro and Agraz، نويسنده , , Alberto and Herrera، نويسنده , , Luis، نويسنده ,
Abstract :
The CB.Hep-1 monoclonal antibody was coupled to CNBr-activated Sepharose CL 4B at three different immobilization scales for purification of recombinant hepatitis B surface antigen. Standard laboratory apparatus to obtain immunosorbents of 1 l (scale I) and 3 l (scale II) as well as a stirrer tank to prepare 6 l immunosorbents (scale III) were used. The binding capacity at scale III was 2- and 1.5-fold higher with respect to the scales II and I, while a reduction in the ligand leakage of 5- and 2-folds was observed. Immunosorbents from scale II showed a significantly reduced adsorption, and an increased ligand leakage. Differences in the coupling efficiency were not observed. Antigen purity eluted from the immunosorbents was always above 85%.
