Title of article
Purification of factor X by hydrophobic interaction chromatography
Author/Authors
Husi، نويسنده , , Holger and Walkinshaw، نويسنده , , Malcolm D.، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2001
Pages
5
From page
367
To page
371
Abstract
Human factor X has been purified to homogeneity by hydrophobic interaction chromatography on phenyl-sepharose. The coagulation protein did not interact with the resin in the presence of 2–3 M NaCl whereas contaminants were retained. This single purification step, in conjunction with classical purification strategies, is a powerful tool in generating high purity factor X and is based on resins which are readily available.
Keywords
factor x
Journal title
Journal of Chromatography B Biomedical Sciences and Applications
Serial Year
2001
Journal title
Journal of Chromatography B Biomedical Sciences and Applications
Record number
1705095
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