Title of article
Heparin column analysis of serum type 5 tartrate-resistant acid phosphatase isoforms
Author/Authors
Igarashi، نويسنده , , Yoshihiko and Lee، نويسنده , , Minako Y. and Matsuzaki، نويسنده , , Shigeru، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2001
Pages
8
From page
269
To page
276
Abstract
The objective of the present study was to develop a specific method for the separation of tartrate-resistant acid phosphatase (TRAP) derived exclusively from osteoclasts. Heparin column-bound TRAP in human serum was separated into three peaks of TRAP activity when eluted with a linear gradient of sodium chloride. The last peak corresponded to TRAP 5b which was first named according to its electrophoretic mobility [Clin. Chem. 24 (1978) 309] and was considered to be derived from osteoclasts [J. Bone Miner. Res. 13 (1998) 683]. The second peak was found to be TRAP 5a. The height of the last peak varied from age to age.
Keywords
enzymes , Tartrate-resistant acid phosphatase
Journal title
Journal of Chromatography B Biomedical Sciences and Applications
Serial Year
2001
Journal title
Journal of Chromatography B Biomedical Sciences and Applications
Record number
1705299
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