Title of article :
Polyamines as cancer markers: applicable separation methods
Author/Authors :
Khuhawar، نويسنده , , M.Y and Qureshi، نويسنده , , G.A، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2001
Abstract :
Spermine, spermidine, putrescine and cadaverine are aliphatic amines widely spread in the human body. Their concentrations together with their acetyl conjugates increase significantly in the biological fluids and the affected tissues of cancer patients. Their concentrations decrease with the improvement in the patient’s condition on multiple therapy. Various chromatographic techniques are frequently used in monitoring concentrations of di- and polyamines in cancer. Among these techniques, thin-layer chromatography and liquid chromatography using pre- or postcolumn derivatization, separating on a reversed-phase or an ion-exchange column are the most commonly used. Besides, high-resolution capillary column gas chromatography (GC) is increasingly used over packed column GC, and in recent years, capillary zone electrophoresis has also gained some importance in polyamine determinations. The review examines the prospects and the limitations of polyamines as cancer markers using chromatographic and electrophoretic techniques.
Keywords :
polyamines , spermidine , spermine , Cadaverine , putrescine
Journal title :
Journal of Chromatography B Biomedical Sciences and Applications
Journal title :
Journal of Chromatography B Biomedical Sciences and Applications