Author/Authors :
El-Masry، نويسنده , , M.M and De Maio، نويسنده , , A and Di Martino، نويسنده , , S and Diano، نويسنده , , N and Bencivenga، نويسنده , , U and Rossi، نويسنده , , S and Grano، نويسنده , , U and Canciglia، نويسنده , , P and Portaccio، نويسنده , , M and Gaeta، نويسنده , , F.S and Mita، نويسنده , , D.G، نويسنده ,
Abstract :
The catalytic behaviour under isothermal conditions of two different membranes loaded with β-galactosidase was investigated. One membrane (M1) was constituted by a nylon sheet grafted with methylmethacrylate by means of chemical grafting. The other, (M2), was prepared by a double chemical grafting: the first one with styrene (Sty) and the second one with methylmethacrylate. Membrane activity was characterized as a function of temperature, pH and substrate concentration. The role of Sty in increasing membrane hydrophobicity has been discussed. Membrane M2 was found to be better suited for employment in non-isothermal bioreactors.
Keywords :
?-Galactosidase , Grafted membranes , Bioreactors , Immobilized enzymes , lactose hydrolysis
