Enzymatic synthesis of three pNP-α-galactobiopyranosides: application of the library of fungal α-galactosidases

The regioselectivity of the transglycosylation reaction catalyzed by extracellular α-galactosidases from filamentous fungi was studied using p-nitrophenyl α-d-galactopyranoside. Regioisomers of p-nitrophenyl α-d-galactobiopyranoside α(1→2), α(1→3) and α(1→6) were isolated and characterized. α-Galactosidases with pronounced regioselectivity towards α-Gal-O-R acceptor were identified.