Author/Authors :
Martino، نويسنده , , A and Schiraldi، نويسنده , , C and Fusco، نويسنده , , S and Di Lernia، نويسنده , , I and Costabile، نويسنده , , T and Pellicano، نويسنده , , T and Marotta، نويسنده , , M and Generoso، نويسنده , , M and van der Oost، نويسنده , , J and Sensen، نويسنده , , C.W and Charlebois، نويسنده , , R.L and Moracci، نويسنده , , M and Rossi، نويسنده , , M and De Rosa، نويسنده , , M، نويسنده ,
Abstract :
An α-glucosidase activity (EC 3.2.1.20) isolated from Sulfolobus solfataricus strain MT-4 was characterised and found of interest at industrial level in the saccharification step of hydrolysis process of starch. The gene encoding for the enzyme was expressed in Escherichia coli BL21 (DE3) with a yield of 87.5 U/g of wet biomass. The recombinant cytosolic enzyme was purified to homogeneity with a rapid purification procedure employing only steps of selective and progressive thermal precipitations with a final yield of 75.4% and a purification of 14.5-fold. The properties of this thermophilic α-glucosidase were compared with those of the α-glucosidase of a commercial preparation from Aspergillus niger used in the starch processing.
Keywords :
?-glucosidase , Starch process , Thermozymes , enzyme purification
