Author/Authors :
Yang، نويسنده , , Sheng and Zhou، نويسنده , , Liping and Tang، نويسنده , , Haixu and Pan، نويسنده , , Jiang and Wu، نويسنده , , Xingjia and Huang، نويسنده , , He and Yuan، نويسنده , , Zhongyi، نويسنده ,
Abstract :
We have used a simple and efficient approach by combining the known functional and structural properties of penicillin G acylase (PGA) from E. coli, and tried to mutate PGA of Bacillus megaterium with the goal of increasing the stability of the enzyme in organic solvents or at acidic pH. The PGA mutants Kβ427A, Kβ430A and Kβ427A/Kβ430A obtained have higher stability in DMF than the wild-type PGA.
