Title of article
Rational design of a more stable penicillin G acylase against organic cosolvent
Author/Authors
Yang، نويسنده , , Sheng and Zhou، نويسنده , , Liping and Tang، نويسنده , , Haixu and Pan، نويسنده , , Jiang and Wu، نويسنده , , Xingjia and Huang، نويسنده , , He and Yuan، نويسنده , , Zhongyi، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2002
Pages
6
From page
285
To page
290
Abstract
We have used a simple and efficient approach by combining the known functional and structural properties of penicillin G acylase (PGA) from E. coli, and tried to mutate PGA of Bacillus megaterium with the goal of increasing the stability of the enzyme in organic solvents or at acidic pH. The PGA mutants Kβ427A, Kβ430A and Kβ427A/Kβ430A obtained have higher stability in DMF than the wild-type PGA.
Keywords
Penicillin G acylase , protein stability , protein engineering
Journal title
Journal of Molecular Catalysis B Enzymatic
Serial Year
2002
Journal title
Journal of Molecular Catalysis B Enzymatic
Record number
1709408
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