Title of article
Optimization of the organic solvent-stable asymmetric hydrogen transfer system of Rhodococcus ruber DSM 44541: an activity-growth study
Author/Authors
Kosjek، نويسنده , , B. and Stampfer، نويسنده , , W. and Glueck، نويسنده , , S.M. and Pogorevc، نويسنده , , M. and Ellmer، نويسنده , , U. and Wallner، نويسنده , , S.R. and Koegl، نويسنده , , M.F. and Poessl، نويسنده , , T.M. and Mayer، نويسنده , , S.F. and Ueberbacher، نويسنده , , B. and Faber، نويسنده , , K. and Kroutil، نويسنده , , W.، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2003
Pages
6
From page
1
To page
6
Abstract
The organic solvent-stable redox-system of Rhodococcus ruber DSM 44541, which allows the efficient oxidation/reduction of sec-alcohols/ketones at the expense of acetone/2-propanol, respectively, as cosubstrate was optimized with respect to a maximum of alcohol dehydrogenase activity during cell growth. Comparison of the fermentation of R. ruber DSM 44541 in shake flasks cultures (1 l flask with 250 ml medium) and in a bioreactor (15 l with 10 l working volume) revealed that the desired organic solvent-stable alcohol dehydrogenase activity reached its maximum during the log phase for the bioreactor. In contrast, in shake flasks the maximum of activity was reached during the stationary phase.
Keywords
Fermentation , Activity-growth , sec-Alcohol dehydrogenase , Rhodococcus ruber
Journal title
Journal of Molecular Catalysis B Enzymatic
Serial Year
2003
Journal title
Journal of Molecular Catalysis B Enzymatic
Record number
1709620
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