Title of article
Monoclonal antibodies against Candida rugosa lipase
Author/Authors
Rahimi، نويسنده , , Hassan and Soro، نويسنده , , Simonetta and Rughetti، نويسنده , , Aurelia and Palocci، نويسنده , , Cleofe and Biffoni، نويسنده , , Mauro and Barachini، نويسنده , , Serena and Taurino، نويسنده , , Federica and Cernia، نويسنده , , Enrico and Frati، نويسنده , , Luigi and Nuti، نويسنده , , Marianna، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2004
Pages
4
From page
71
To page
74
Abstract
We generated monoclonal antibodies (MoAbs) BF11 and VNH9 against Candida rugosa lipase (CRL), one of the most widely used lipolytic enzymes. The antibodies, both of the IgG1 isotype recognize specie specific and distinct antigenic determinants shared by different CRL isoforms present both in the native and in the denaturated form of the enzyme. The CRL enzyme maintained its enzymatic activity even when immunocomplexed with MoAb BF11 and VNH9. These novel reagents are of interest for their possible application as immobilization and purification systems, and during fermentation processes.
Keywords
Candida rugosa lipase , Microbial lipase , IgG1 isotype
Journal title
Journal of Molecular Catalysis B Enzymatic
Serial Year
2004
Journal title
Journal of Molecular Catalysis B Enzymatic
Record number
1710067
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