Title of article
Characterization of GDP-mannose pyrophosphorylase from Escherichia coli O157:H7 EDL933 and its broad substrate specificity
Author/Authors
Yang، نويسنده , , Yung-Hun and Kang، نويسنده , , Young-Bok and Lee، نويسنده , , Kwang-Won and Lee، نويسنده , , Tek-Hyung and Park، نويسنده , , Sung-Soo and Hwang، نويسنده , , Bum-Yeol and Kim، نويسنده , , Byung-Gee، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2005
Pages
8
From page
1
To page
8
Abstract
GDP-mannose pyrophosphorylase gene (ManC) of Escherichia coli (E. coli) O157 was cloned and expressed as a highly soluble protein in E. coli BL21 (DE3). The enzyme was subsequently purified using hydrophobic and ion exchange chromatographies. ManC showed very broad substrate specificities for four nucleotides and various hexose-1-phosphates, yielding ADP-mannose, CDP-mannose, UDP-mannose, GDP-mannose, GDP-glucose and GDP-2-deoxy-glucose.
Keywords
ESI-MS , NDP-mannose , Substrate Specificity , GDP-mannose pyrophosphorylase
Journal title
Journal of Molecular Catalysis B Enzymatic
Serial Year
2005
Journal title
Journal of Molecular Catalysis B Enzymatic
Record number
1710704
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