Biofabrication of gold nanoparticles and its biocompatibility in human breast adenocarcinoma cells (MCF-7)

In this paper, a simple and environmentally friendly method for synthesis of gold nanoparticles (AuNPs) using culture supernatant of Bacillus flexus as reductants and stabilizers is reported. The prepared AuNPs were characterized by various analytical techniques. UV–visible spectroscopy, X-ray diffraction (XRD), energy dispersive X-ray spectroscopy (EDS) results confirmed that Au3+ ions reduced into Au0. Fourier-transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS) measurements confirmed that the chemical surface structure of AuNPs. TEM images showed the size and morphology of AuNPs. Moreover, the toxicity studies suggested that AuNPs were neither toxic nor inhibitory to human breast cancer cells (MCF-7).