Author/Authors :
Chen، نويسنده , , Ying and Xiao، نويسنده , , Chen-peng and Chen، نويسنده , , Xiaoyun and Yang، نويسنده , , Li-wei and Qi، نويسنده , , Xin and Zheng، نويسنده , , Jianfeng and Li، نويسنده , , Mingchun and Zhang، نويسنده , , Jun، نويسنده ,
Abstract :
In this work, a novel method to prepare cross-linked enzyme aggregates (CLEA) in a water-in-oil emulsion was reported. Spherical CLEA of recombinant trehalose synthase (TreS) from Meiothermus rubber was obtained through the emulsion based process. By varying the experimental parameters, the optimum conditions for the preparation of spherical CLEA were determined. The optimum catalytic temperature and thermal stability of spherical CLEA were enhanced significantly compared with the free enzyme. Spherical CLEA was also more tolerant to metal ions and chemical denaturants. Optical and scanning electron microscopy analysis showed that instead of the amorphous clumping shape of conventionally prepared CLEA, spherical CLEA has a more structured ball-like appearance with a size of 20–60 μm in diameter that could be easily separated.
