Improvement of the thermal stability and aldehyde tolerance of deoxyriboaldolase via immobilization on nano-magnet material

EcoDERA2 as efficiently immobilized on GA-APTES-NSM11 ES-NSM: glutaraldehyde-(3-aminopropyl)triethoxysilane nano-magnet material a yield of over 75% After incubation of the free EcoDERA in 300 mM acetaldehyde for 3 h, no activity was observed, while in contrast, the EcoDERA-GA-APTES-NSM22 A immobilized on glutaraldehyde-(3-aminopropyl)triethoxysilane nano-magnet material ned more than 60% of its activity after a 3 h incubation. The EcoDERA-GA-APTES-NSM possessed 65.7% of its initial activity after incubation at 80 °C for 10 min, whereas, the free enzyme lost almost all of its activity under these conditions. The immobilized enzyme can be easily separated from the reaction system by using only a magnetic-iron.These results suggest the potential utility of GA-APTES-NSM as an immobilization support for EcoDERA.