Biosynthesis of myristyl serinate by immobilized Candida antarctica lipase in two-phase system

Myristyl serinate was firstly enzyme-synthesized from myristyl alcohol and l-serine in selected solvents (single and two-phase solvents). The effects of reaction system, two-phase system composition, temperature, pH, and reaction time on myristyl serinate synthesis were investigated. The results showed that two-phase solvent reaction system could significantly increase the enzymatic conversion ratio and the optimal ratio of two-phase solvent was 80:20 (dichloromethane:phosphate buffer). The optimal conditions for myristyl serinate enzymatic synthesis were substrate molar ratio of 1:1, Novozym 435 load of 5% (w/w), a solvent dosage of 20 ml, 30 °C and reaction time of 36 h. Under these conditions, 72.25% of myristyl alcohol could be converted to myristyl serinate, which was purified through column chromatography and identified by HPLC/IR/LC-MS/NMR.