Magnetic microparticulate carriers with immobilized selective ligands in DNA diagnostics

Magnetic poly(2-hydroxyethyl methacrylate)- and poly(glycidyl methacrylate)-based microparticles were prepared by dispersion polymerization in the presence of iron oxide nanoparticles, both commercial and laboratory-made. The polymerization was highly sensitive to even subtle changes in the various reaction parameters involved in the process. The size of the final microparticles was determined by the composition of the dispersion medium (e.g. water/ethanol ratio, monomer concentration at the moment of phase separation, stabilizer concentration, initiator type and concentration, polymerization temperature). Several DNA applications of developed microparticles were described, among others RNA and DNA degradation and Salmonella cell magnetic separation by RNase A and DNase I and anti-Salmonella or proteinase K immobilized on developed magnetic carriers. The sensitivity of polymerase chain reaction (PCR) in cell detection was negatively affected by some magnetic carriers and compounds used in their preparation. Carboxyl group-containing magnetic microparticles were prepared for isolation of genomic DNA from cell lysate in the presence of poly(ethylene glycol) and sodium chloride.