Author/Authors :
Vimal، نويسنده , , S. and Abdul Majeed، نويسنده , , S. and Taju، نويسنده , , G. and Nambi، نويسنده , , K.S.N. and Sundar Raj، نويسنده , , N. and Madan، نويسنده , , N. and Farook، نويسنده , , M.A. and Rajkumar، نويسنده , , T. and Gopinath، نويسنده , , D. and Sahul Hameed، نويسنده , , A.S.، نويسنده ,
Abstract :
The present study examines the use of CS/TPP nanoparticles for gene delivery in different tissues of shrimp through oral route. The viral gene of WSSV was used to construct DNA vaccines using pcDNA 3.1, a eukaryotic expression vector and the constructs were named as pVP28. The CS/TPP nanoparticles were synthesized by ionic gelation process and these particles were characterized. The structure and morphology of the nanoparticles were studied by field emission scanning electron microscopy (FE-SEM) and FTIR (Fourier Transform Infrared Spectra). The cytotoxicity of CS/TPP nanoparticles was evaluated by MTT assay using fish cell line. The expression of gene was confirmed by Immuno-dot blot, ELISA and RT-PCR analyses. The results indicate that DNA can be easily delivered into shrimp by feeding with CS/TPP nanoparticles.
Keywords :
Immuno-dot blot , CS/TPP nanoparticles , In vivo gene expression , pVP28 DNA , Real-Time PCR
