Genetic Diversity Analysis of Cluster Bean [Cyamopsis tetragonoloba (L.) Taub] Genotypes Using RAPD and ISSR Markers

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In the present study, random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) markers were used to estimate genetic diversity and relationships among 35 cluster bean [Cyamopsis tetragonoloba (L.) Taub] genotypes. For RAPD analysis, 20 random primers were used which amplified 164 bands, 147 of which were polymorphic with an average polymorphism of 90.3%. The amplified products varied in size from 250 to 2,050 bp. For ISSR analysis, 10 primers were used which produced 105 bands, 102 of which were polymorphic (97%). The size of amplified bands ranged from 450 to 3,500 bp. The efficiency of primers in generating sufficient information for genetic diversity analysis was computed using discriminatory power (Dj), which ranged from 0.40 to 0.98 for RAPDs and 0.44 to 0.99 for ISSRs. Jaccard similarity coefficients were used to estimate the genotypic association with each other, which varied from 0.38 to 0.91 for RAPDs and from 0.20 to 0.88 for ISSRs. Cluster analysis indicated that all 35 genotypes could be distinguished by both RAPD and ISSR markers. Both of the methods (RAPD and ISSR) showed significant correlation (r= 0.69), implying their equal importance in cluster bean diversity analysis.