Author/Authors :
Basak، Geetanjali نويسنده 1Environmental Biotechnology Division, School of Bio- Sciences and Technology, VIT University, Vellore, Tamil Nadu, India , , V، Lakshmi نويسنده 1Environmental Biotechnology Division, School of Bio- Sciences and Technology, VIT University, Vellore, Tamil Nadu, India , , Chandran، Preethy نويسنده School of Chemical and Biotechnology, SASTRA University, Thanjavur, Tamil Nadu, India , , Das، Nilanjana نويسنده 1Environmental Biotechnology Division, School of Bio- Sciences and Technology, VIT University, Vellore, Tamil Nadu, India ,
Abstract :
Background: Present study deals with the removal of Zn(II) ions from effluent using yeast biofilm formed on
gravels.
Methods: The biofilm forming ability of Candida rugosa and Cryptococcus laurentii was evaluated using XTT (2,3-bis
[2-methoxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxanilide) reduction assay and monitored by scanning
electron microscopy (SEM), and Confocal laser scanning microscopy (CLSM). Copious amount of extracellular
polymeric substances (EPS) produced by yeast species was quantified and characterized by Fourier transform
infrared spectroscopy (FT-IR).
Results: Yeast biofilm formed on gravels by C. rugosa and C. laurentii showed 88% and 74.2% removal of Zn(II) ions
respectively in batch mode. In column mode, removal of Zn(II) ions from real effluent was found to be 95.29% by C.
rugosa biofilm formed on gravels.
Conclusion: The results of the present study showed that there is a scope to develop a cost effective method for
the efficient removal of Zn(II) from effluent using gravels coated with yeast biofilm.
