Title of article :
Influence of β-tricalcium phosphate granule size and morphology on tissue reaction in vivo
Author/Authors :
Ghanaati، نويسنده , , Shahram and Barbeck، نويسنده , , Mike and Orth، نويسنده , , Carina and Willershausen، نويسنده , , Ines and Thimm، نويسنده , , Benjamin W. and Hoffmann، نويسنده , , Christiane and Rasic، نويسنده , , Angela and Sader، نويسنده , , Robert A. and Unger، نويسنده , , Ronald E. and Peters، نويسنده , , Fabian and Kirkpatrick، نويسنده , , C. James، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2010
Pages :
12
From page :
4476
To page :
4487
Abstract :
In this study the tissue reaction to five different β-tricalcium phosphate (β-TCP)-based bone substitute materials differing only in size, shape and porosity was analyzed over 60 days, at 3, 10, 15, 30 and 60 days after implantation. Using the subcutaneous implantation model in Wistar rats both the inflammatory response within the implantation bed and the resulting vascularization of the biomaterials were qualitatively and quantitatively assessed by means of standard and special histological staining methods. The data from this study showed that all investigated β-TCP bone substitutes induced the formation of multinucleated giant cells. Changes in size, shape and porosity influenced the integration of the biomaterials within the implantation bed and the formation of tartrate-resistant acid phosphatase (TRAP)-positive and TRAP-negative multinucleated giant cells, as well as the rate of vascularization. While a high porosity generally allowed cell and fiber in-growth within the center of the bone substitute, a lower porosity resulted in a mosaic-like integration of the materials, with the granules serving as place holders. The number of multinucleated giant cells located in the implantation bed positively correlated with the vascularization rate. These data emphasize that all biomaterials investigated were capable of inducing the formation of TRAP-positive multinucleated giant cells as a sign of biomaterial stability. Furthermore, these cells directly influenced vascularization by secretion of vascular endothelial growth factor (VEGF), as well as other chemokines. Based on these findings, the role of multinucleated giant cells in the foreign body reaction to biomaterials might need to be reconsidered. This study demonstrates that variations in the physical properties of a bone substitute material clearly influence the (extent of the) inflammatory reaction and its consequences.
Keywords :
Cerasorb , Bioactivity , ?-TCP , Vascularization , Osteoclast-like cells
Journal title :
Acta Biomaterialia
Serial Year :
2010
Journal title :
Acta Biomaterialia
Record number :
1754447
Link To Document :
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