Author/Authors :
Rafiee، Behnam نويسنده Department of Microbiology, Islamic Azad University, Qom Branch, Qom ,Iran Rafiee, Behnam , Mosavari، Nader نويسنده Department of Microbiology, Razi Vaccine & Serum Research Institute, Tehran University of Medical Sciences, Tehran, Iran Mosavari, Nader , Ghani، Sepideh نويسنده Young Researchers Club and Elite, Arak Branch, Islamic Azad University, Arak, Iran Ghani, Sepideh , Sadeghi، Davoud نويسنده Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran Sadeghi, Davoud , Keshavarz، Rouholah نويسنده Razi Vaccine and Serum Research Institute, Karaj, Iran Keshavarz, Rouholah , Tadayon، Keyvan نويسنده Razi Vaccine and Serum Research Institute, Karaj, Iran Tadayon, Keyvan
Abstract :
Background: Mycobacterium bovis is the causative agent of bovine tuberculosis and belongs to the Mycobacterium tuberculosis complex. M. bovis usually carries only one or a few copies of the insertion sequence IS6110 in its genome. The aim of this study was evaluation of copy number of IS6110 in M. bovis isolates by restriction fragment length polymorphism (RFLP) method.
Materials and Methods: In this experimental study, 25 lymph node specimens of tuberculin-positive cattle were collected and cultured by standard methods, afterward genomic DNA was extracted by chloroform-isoamyl alcohol. Genetic studies were conducted by Pvull and DNA hybridization with IS6110.
Results: Two isolates displayed more than of 4 copies of IS6110 by RFLP (IS6110-RFLP) method.
Conclusion: The results of this study are unique and specific in Iran but reported in the world rarely. Therefore the new strains of M. bovis imported to Iran from other countries of the world.
