Title of article :
Human corneal endothelial cell sheets for transplantation: Thermo-responsive cell culture carriers to meet cell-specific requirements
Author/Authors :
Teichmann، نويسنده , , J. and Valtink، نويسنده , , M. and Gramm، نويسنده , , S. and Nitschke، نويسنده , , M. and Werner، نويسنده , , C. and Funk، نويسنده , , R.H.W. and Engelmann، نويسنده , , K.، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2013
Pages :
9
From page :
5031
To page :
5039
Abstract :
Corneal endothelial diseases lead to severe vision impairment, motivating the transplantation of donor corneae or corneal endothelial lamellae, which is, however, impeded by endothelial cell loss during processing. Therefore, one prioritized aim in corneal tissue engineering is the generation of transplantable human corneal endothelial cell (HCEC) layers. Thermo-responsive cell culture carriers are widely used for non-enzymatic harvest of cell sheets. The current study presents a novel thermo-responsive carrier based on simultaneous electron beam immobilization and cross-linking of poly(vinyl methyl ether) (PVME) on polymeric surfaces, which allows one to adjust layer thickness, stiffness, switching amplitude and functionalization with bioactive molecules to meet cell type specific requirements. The efficacy of this approach for HCEC, which require elaborate cell culture conditions and are strongly adherent to the substratum, is demonstrated. The developed method may pave the way to tissue engineering of corneal endothelium and significantly improve therapeutic options.
Keywords :
Thermo-responsive polymers , electron beam , Cell culture , Corneal endothelium , Biomolecular functionalization
Journal title :
Acta Biomaterialia
Serial Year :
2013
Journal title :
Acta Biomaterialia
Record number :
1756800
Link To Document :
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