Author/Authors :
Keita، نويسنده , , Bineta and Essaadi، نويسنده , , Khalid and Nadjo، نويسنده , , Louis and Desmadril، نويسنده , , Michel، نويسنده ,
Abstract :
The kinetics of NADH oxidation by 3 Dawson-type mixed heteropolyanions were studied in buffered aqueous pH = 7 medium, by the stopped flow technique and UV-visible spectroscopy. The log of k was a linear function of the E0 of the first redox systems of the heteropolyanions with a slope of 16.5 V−1. The results indicate that, in the present case, the oxidation of NADH proceeds by a multistep mechanism involving initial rate-limiting one-electron transfer. An estimate of the E0 value for the one-electron NADH/NADH+· couple has been obtained.
