Author/Authors :
Konopasek، نويسنده , , Ivo and Kvasnicka، نويسنده , , Peter and Herman، نويسنده , , Petr and Linnertz، نويسنده , , Holger and Obsil، نويسنده , , Tomas and Vecer، نويسنده , , Jaroslav and Svobodova، نويسنده , , Jaroslava and Strzalka، نويسنده , , Kazimierz and Mazzanti، نويسنده , , Laura and Amler، نويسنده , , Evzen، نويسنده ,
Abstract :
Fluorescence decay of 1,6-diphenyl-1,3,5-hexatriene (DPH) was found to be bicomponental in lipid bilayers and isotropic solvents. Mean lifetime values of both components correlated with the permittivity of the solvents. Decomposition of time-resolved spectra of DPH in phospholipid vesicles revealed a significant red shift of the spectrum of the short-lived component with respect to the spectrum of the long-lived component. This indicated that part of DPH was in a hydrophilic environment, which was supported by fluorescence energy transfer experiments. We showed that the increased fraction of the short-lived component in lipid bilayers originates from the population of DPH at the membrane–water interface.
