Author/Authors :
Rolinski، نويسنده , , O.J. and Birch، نويسنده , , D.J.S. and McCartney، نويسنده , , L.J. and Pickup، نويسنده , , J.C، نويسنده ,
Abstract :
The near-infrared fluorescence resonance energy transfer kinetics of the phycobiliprotein allophycocyanin (APC) to malachite green (MG) have been investigated. A model is proposed to account for the fluorescence decay whereby MG binds to APC with a donor–acceptor site distribution which can be best described by 2D quenching kinetics. The results highlight a potential fallacy when interpreting the dimensionality of complex systems or the location of binding sites from Förster decay kinetics. The use of APC in trans-dermal measurements is proposed.
