Title of article
Two-photon excitation and time-resolved fluorescence: I. The proper response function for analysing single-photon counting experiments
Author/Authors
Habenicht، نويسنده , , Anja and Hjelm، نويسنده , , Johan and Mukhtar، نويسنده , , Emad and Bergstrِm، نويسنده , , Fredrik and Johansson، نويسنده , , Lennart B.-إ.، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2002
Pages
9
From page
367
To page
375
Abstract
An accurate instrumental response function is needed to deconvolute fluorescence data obtained by time-correlated single-photon counting (TCSPC) upon multi-photon excitation. Hitherto the response function was obtained by measuring Rayleigh scattering (RS) from colloidal solutions, as is also used in one-photon excited fluorescence. We show that hyper Rayleigh scattering (HRS) provides a better choice for deconvolution of fluorescence decays, as obtained by TCSPC and two-photon excitation (TPE). The one- and two-photon response functions were monitored as RS and HRS from colloidal gold particles at 800 and 400 nm, respectively.
Journal title
Chemical Physics Letters
Serial Year
2002
Journal title
Chemical Physics Letters
Record number
1779937
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