Author/Authors :
Agathe and Changenet-Barret، نويسنده , , Pascale and Espagne، نويسنده , , Agathe and Katsonis، نويسنده , , Nathalie Dray-Charier، نويسنده , , Sandrine and Baudin، نويسنده , , Jean-Bernard and Jullien، نويسنده , , Ludovic and Plaza، نويسنده , , Pascal and Martin، نويسنده , , Monique M، نويسنده ,
Abstract :
Cis–trans photoisomerization of a photoactive yellow protein chromophore model, the deprotonated trans S-phenyl thio-p-hydroxycinnamate, is studied in aqueous solution by subpicosecond transient absorption and gain spectroscopy. The excited-state deactivation is found to involve the formation, in 1.7 ps, of an intermediate state which decays in 2.8 ps. A persistent bleaching signal is observed at longer times indicating that the excited state not only relaxes to the ground state but also partly forms a stable photoproduct, possibly the cis isomer. This behavior is analogous to that of the native photoactive yellow protein.
