Author/Authors :
Ji، نويسنده , , Xiaohui and Xu، نويسنده , , Shuping and Wang، نويسنده , , Lianying and Liu، نويسنده , , Min and Pan، نويسنده , , Kai-Tao Yuan، نويسنده , , Hang and Ma، نويسنده , , Lan and Xu، نويسنده , , Weiqing and Li، نويسنده , , Jinghong and Bai، نويسنده , , Yubai and Li، نويسنده , , Tiejin، نويسنده ,
Abstract :
Au/Ag core-shell nanoparticles labeled by monoclonal antibody and surface-enhanced Raman scattering (SERS) probes were used for immunoassay analysis. Mouse polyclonal antibodies against hepatitis B surface antigen (mouse Anti-HBsAg PAb) were covalently immobilized on the silicon chips modified by a self-assembled monolayer of (3-amino-propyl) trimethoxysilane via glutaraldehyde (GA) activation. The Au/Ag core-shell nanoparticles were labeled by the mouse monoclonal antibody against hepatitis B surface antigen (mouse Anti-HBsAg MAb) and the probe molecule, p-mercaptobenzoic acid (MBA). Immunoreactions between the labeled Au/Ag core-shell nanoparticles and the corresponding antigens captured by the Anti-HBsAg PAb on the silicon chips were investigated by SERS.
Keywords :
Immunoassay , Au/Ag core-shell nanoparticles , Antigen , Antibody , SERS
