Title of article :
Taenia solium: Germinal Cell Precursors in Tapeworms Grown in Hamster Intestine
Author/Authors :
Willms، نويسنده , , Kaethe and Merchant، نويسنده , , Marie Thêrese and Gَmez، نويسنده , , Margarita and Robert، نويسنده , , Lilia، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2001
Abstract :
Background
revious study, it was shown that growth of evaginated metacestodes occurs in the germinative tissue of the neck by duplication of somatic stem cells. In these specimens, it was not possible to find the mitotic figures required to demonstrate duplication of germ cell lines.
s
solium strobilae were collected from the intestinal lumen of outbred hamsters infected orally with 10 metacestodes dissected from naturally infected pigs. Animals were anesthetized 1–10 days postinfection, the small intestine excised, submerged in PBS, and cut open longitudinally. Live Taenias were incubated for 6–8 h in medium containing colchicine or 3H-thymidine, washed, and embedded for electron microscopy. For light microscopy and autoradiography, longitudinal sections were cut from whole blocks and mounted on glass slides. A population of large cells without nuclear membranes and containing discrete aggregates of chromatin were observed apposed to myofibrils in the germinative tissue of the neck. These cells were confirmed by electron microscopy as metaphase mitotic figures, with chromosomes attached to a microtubular spindle, embedded in cytoplasm, without a nuclear membrane, and with characteristic centrioles.
s
apeworms in which 3H-thymidine was injected directly into the worm tissue by microsyringe were positive by autoradiography, demonstrating that in contrast to evaginated metacestodes, intestinal worms do not transport thymidine across the tegument.
sions
sults show that differentiating T. solium worms have a subset of stem cells that require passage through a mammalian host to go into mitosis, and that tapeworms grown in an experimental animal do not take up 3H-thymidine in vitro.
Keywords :
Taenia solium , Cestode , Stem cells , germinal cells , Ultrastructure , autoradiography
Journal title :
Archives of Medical Research
Journal title :
Archives of Medical Research