Sequence analysis of canine p53 in the region of exons 3–8

An 828 base pair region of canine wild-type p53, corresponding to codons 25 through 312 in the human p53 gene, was directly sequenced from asymmetric polymerase chain reaction (PCR) products. The deduced amino acid sequence of the dog was 86%, 73% and 83% homologous to that of the cat, mouse and human proteins, respectively. In the evolutionarily conserved domains II through V, the canine sequence approached 100% homology with the human sequence. The region sequenced encompasses exons 3–8 where the majority of mutations have been identified in neoplastic specimens from human patients.