Author/Authors :
Hotta، نويسنده , , Tsukasa and Tanimura، نويسنده , , Hiroshi and Yamaue، نويسنده , , Hiroki and Iwahashi، نويسنده , , Makoto and Tani، نويسنده , , Masaji and Tsunoda، نويسنده , , Takuya and Noguchi، نويسنده , , Kohei and Mizobata، نويسنده , , Shizuma and Terasawa، نويسنده , , Hiroshi، نويسنده ,
Abstract :
We examined the synergistic effects of tamoxifen (TAM) and cepharanthine (CEP) for doxorubicin (DOX) sensitivity using MTT assay. The augmentation of DOX sensitivity by TAM and CEP was significantly correlated with the P-glycoprotein expression. The cytotoxic effect of DOX with TAM and CEP was significantly higher than that of DOX alone, or DOX with TAM, and this synergistic effect was dominant in cell lines with high expression of P-glycoprotein. It was also examined that the intracellular concentration of DOX was increased in combined exposure of TAM and CEP, compared with the exposure of TAM, because TAM and CEP promoted the influx and inhibited the efflux of DOX. Thus, TAM and CEP might be able to circumvent DOX-resistance for treatment in cancer patients.
Keywords :
Multidrug resistance , doxorubicin , Tamoxifen , P-GLYCOPROTEIN , Cepharanthine
