Cloning and characterization of a cDNA fragment coding β-casein-like protein preferentially expressed in cervical adenocarcinoma cell line CAC-1

We generated new murine monoclonal antibodies against bovine β-casein. One of eight new monoclonal antibodies generated, 12G2, specifically reacted not only with β-casein, but also with human cervical adenocarcinoma cell line CAC-1. To identify the gene encoding the putative protein (BCLP) defined by monoclonal antibody (mAb) 12G2, a complementary DNA library was constructed. We obtained the inserted cDNA fragment that showed high homology to reported DNA sequences of unknown function in normal human placenta. We think that BCLP was overexpressed or changed its structure by carcinogenesis.