Inhibition of colonization and cell–matrix adhesion after nm23-H1 transfection of human prostate carcinoma cells

A human gene, nm23-H1, has been known as a metastasis suppressor in many tumor cells. The cellular mechanisms by which the nm23-H1 protein may directly or indirectly modulate the metastatic phenotype are not yet known. In this study the phenotypic effect of transfection of nm23-H1 cDNA into the human DU 145 prostate carcinoma cell line was examined. Despite similar growth rates, the nm23-H1-transfected lines displayed decreased colonization in soft agar and adhesion to extracellular matrix components when compared with the control transfected line. The results suggest that the nm23-H1 gene product suppresses the metastatic potential of prostate carcinoma cells by inhibiting their ability in anchorage-independent growth and extracellular matrix adhesion.