Streptococcus oralis previously identified as uncommon ‘Streptococcus sanguis’ in Behçetʹs disease

The relation between the biochemical and serological properties of ‘Streptococcus sanguis’ was studied to characterize the strains isolated from dental plaque of patients with Behçetʹs disease and controls. Seven reference and 100 clinical strains preserved by the Behçetʹs Disease Research Committee of Japan were identified using established criteria and differentiated with antisera against Strep. oralis ATCC 10557, Strep. sanguis ATCC 10556 and ‘Strep. sanguis’ ST7, compatible with the criteria. Uncommon serovars (serotypes) KTH-1 (=ATCC 49298), KTH-2 (=ATCC 49296), KTH-3 (=ATCC 49295), KTH-4 (=ATCC49297) and B220 (serovar KTH-1) with both IgAl protease and neurominidase (sialidase) were identified as Strep. oralis, whereas common serovars ST3 with IgA1 protease alone and ST7 without both enzymes were identified as Strep. sanguis and Strep. gordonii, respectively. Isolates previously ranked as uncommon serovars were identified as Strep. oralis, whereas the rest ranked as common serovars were identified as the same species as those of the grouping strains. A soft-agar technique was available for species identification except for Strep. oralis serovars KTH-1 reacting with the antiserum against Strep. gordonii ST7. The frequency of isolation of Strep. oralis was higher in Behçetʹs disease (52%) than in controls (38%), but no difference was observed between the properties of the two groups of isolates. Strep. oralis virulence factors may be involved in breach the mucosal barrier in patients with specific reactivity to these antigens and inducing Behçetʹs disease.