The activity of caspase-3-like proteases is elevated during the development of colorectal carcinoma

Activated caspase-3-like proteases promote apoptotic cell death by cleaving cellular substrates. Caspase-3-like activity was measured in colonic carcinomas and in matched normal colonic mucosa from 31 patients and was significantly elevated in 25/31 colonic carcinomas and adenomas when compared to normal mucosa (P<0.0001). Caspase-3-like activity was much higher in normal mucosa and tumours of female subjects than of males (P<0.0001). No correlation was obtained between caspase-3-like activity and location of the tumour, tumour grade, stage, or patient age. The marked increase in caspase-3-like activity in colorectal carcinomas may reflect an increase in the proportion of cells undergoing spontaneous apoptosis.