Title of article :
Expression of membrane-type 1 matrix metalloproteinase (MT1-MMP) on prostate cancer cell lines
Author/Authors :
Nagakawa، نويسنده , , Osamu and Murakami، نويسنده , , Koji and Yamaura، نويسنده , , Takeshi and Fujiuchi، نويسنده , , Yasuyoshi and Murata، نويسنده , , Jun and Fuse، نويسنده , , Hideki and Saiki، نويسنده , , Ikuo، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2000
Abstract :
Membrane-type metalloproteinase-1 (MT1-MMP) is a transmembrane metalloproteinase, which activates proMMP-2 and expressed on the cell surface in many invasive cancer cells. We investigated the expression of MT1-MMP in prostate cancer cell lines. MT1-MMP protein and mRNA were expressed in PC-3, DU-145 and TSU-pr1 cells (androgen-independent prostate cancer cell lines), but in LNCaP cells (androgen-dependent prostate cancer cell line). MT1-MMP protein was negative and mRNA was low to detect by RT-PCR. Cell lysate of PC-3 cleaved proMMP-2 to the active form. In addition, both hepatocyte growth factor (HGF) and gastrin-releasing peptide (GRP) increased Matrigel invasion and induced the expression of MT1-MMP protein in DU-145 prostate cancer cells. These results suggest that MT1-MMP is indeed the tumor-specific activator of proMMP-2 in androgen-independent prostate cancer cells and plays an important role in the invasive properties of prostate cancer cells.
Keywords :
Prostate carcinoma , Hepatocyte growth factor , gastrin-releasing peptide , Membrane-type metalloproteinase-1 , Invasion
Journal title :
Cancer Letters
Journal title :
Cancer Letters