Author/Authors :
Li، نويسنده , , Zhong-you and Wang، نويسنده , , You-jie and Song، نويسنده , , Jian-ping and Kataoka، نويسنده , , Hideki and Yoshii، نويسنده , , Shigeto and Gao، نويسنده , , Chang-ming and Wang، نويسنده , , Ya-ping and Zhou، نويسنده , , Jian-nong and Ota، نويسنده , , Satoshi and Tanaka، نويسنده , , Masamitsu and Sugimura، نويسنده , , Haruhiko، نويسنده ,
Abstract :
β-PIX, a newly identified p21-activated kinase (PAK)-interacting exchange factors (PIX), encodes a guanine nucleotide exchange factor for Rho guanosine triphosphatases. Characterization of β-PIX gene was performed using the BAC Library method. The β-PIX gene has 17 exons and an A/T polymorphism at the 32nd base upstream of the intron/exon junction of exon 7. The frequencies of genotypes A/T, A/A and T/T were 23.6% (13/55), 72.7% (40/55) and 3.6% (2/55), respectively; these frequencies are in Hardy–Weinberg equilibrium. Two out of 14 informative tumors (14.3%) were shown to have lost their heterozygosity at this locus, but no mutations in the remaining alleles were detected. In addition, we examined the gene-expression profile in another set of 30 gastric samples, but no significant over-expression of either the β-PIX gene or the α-PIX gene was found. Though the β-PIX gene has been speculated to potentially have tumor-related biological characteristics, the findings of the present study suggest that the involvement of β-PIX gene in human gastric carcinogenesis is minimal.
Keywords :
Loss of Heterozygosity , genomic structure , Gene expression , Gastric cancer , p21-Activated kinase-interacting exchange factors , Polymorphism
