Author/Authors :
Vitali، نويسنده , , Liliana and Yakisich، نويسنده , , J.Sebastian and Vita، نويسنده , , Marina Fernanda and Fernandez، نويسنده , , Alejandro and Settembrini، نويسنده , , Liliana and Siden، نويسنده , , إke and Cruz، نويسنده , , Mabel and Carminatti، نويسنده , , Hector and Casas، نويسنده , , Oscar and Idoyaga Vargas، نويسنده , , Victor، نويسنده ,
Abstract :
The effect of roscovitine, a purine analogue and cyclin dependent kinase inhibitor, on DNA synthesis rate in tissue mini-units obtained from human cervical cancers was investigated. Roscovitine (100 μM) gave a DNA synthesis rate inhibition by 61% (P<0.0001; range 23–93%) within 30 min of incubation. This inhibitory effect was concentration-dependent. The results suggest that the inhibition of tumor DNA synthesis rate is due to a direct effect on the DNA synthesis machinery via presently unknown mechanisms. In addition, the potential application of CDKs inhibitors as preventive agents is discussed.
Keywords :
cervical cancer , Roscovitine , cyclin dependent kinases , DNA synthesis
