The serine proteinase thrombin promotes migration of human renal carcinoma cells by a PKA-dependent mechanism

In this study, we demonstrated that thrombin activates protein kinase C (PKC), mitogen activated protein kinases (MAP kinases), transcription factor nuclear factor-κB (NF-κB), and cAMP-dependent protein kinase (PKA) in the human renal carcinoma cell line A-498. In addition, it enhanced the migratory capacity, but had no effect on the proliferation of A-498 cells. The effect of thrombin on migration could be blocked by the PKA inhibitor H-89 but was not influenced by inhibition of PKC, MAP kinases or NF-κB. We concluded, that thrombin acts as a regulator on human A-498 renal carcinoma cell migration including PKA.