Title of article :
Effect of iron on the dissolution of bovine enamel powder in vitro by carbonated beverages
Author/Authors :
Kato، نويسنده , , Melissa Thiemi and Maria، نويسنده , , Andrea Gutierrez and Sales-Peres، نويسنده , , Sيlvia Helena de Carvalho and Buzalaf، نويسنده , , Marيlia Afonso Rabelo، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2007
Abstract :
Objectives
m of this study was to evaluate, in vitro, the effect of iron on the dissolution of bovine enamel powder, when added to two carbonated beverages.
ed enamel was produced by griding enamel fragments of bovine incisor in a steel pestle and mortar. Particles between 75 and 106 μm were selected using appropriated meshes. At time zero, the carbonated beverage (Coke® or Sprite Zero®) was added to powdered enamel (1 mg enamel powder/10 μL of beverage) and vortexed for 30 s. The sample was immediately centrifuged (11,000 rpm) for 30 s and the supernatant was removed at 1 min 40 s. This procedure was repeated five times with the beverage containing increasing ferrous sulphate concentrations (1.25, 2.5, 5, 10, 15, 30 and 60 mmol/L). The phosphate released in the medium was analysed spectrophotometrically. Data were analysed using ANOVA and Tukeyʹs test (p < 0.05).
s
ron at 30 and 60 mmol/L was added to Coke®, a significant reduction in the dissolution of powdered enamel was observed when compared to control (11 and 17%, respectively), while lower iron concentrations did not have any effect on enamel powder dissolution. Regarding Sprite Zero®, iron concentrations up to 10 mmol/L had no significant effect, while higher concentrations significantly increased enamel powder dissolution.
sions
sults suggest that iron can interfere with the dissolution of dental enamel powder in the presence of acidic beverages and the type of acid in these beverages seems to modulate this effect.
Keywords :
Tooth Erosion , Demineralisation , Iron , Dental enamel
Journal title :
Archives of Oral Biology
Journal title :
Archives of Oral Biology