Author/Authors :
Ito، نويسنده , , Seiji and Nakanishi، نويسنده , , Hayao and Hirai، نويسنده , , Takashi and Kato، نويسنده , , Tomoyuki and Kodera، نويسنده , , Yasuhiro and Feng، نويسنده , , Zhang and Kasai، نويسنده , , Yasushi and Ito، نويسنده , , Katsuki and Akiyama، نويسنده , , Seiji and Nakao، نويسنده , , Akimasa and Tatematsu، نويسنده , , Masae، نويسنده ,
Abstract :
We applied novel real-time reverse transcriptase-polymerase chain reaction (RT-PCR) with a LightCycler for quantitative detection of carcinoembryonic antigen (CEA) mRNA expressing tumor cells in the peripheral blood of colorectal cancer patients. Analysis of peripheral blood samples from 99 potentially curative colorectal cancer patients revealed a significantly higher mean CEA mRNA value in post-operative bloods (18.71) than in pre-operative blood (1.03) (P=0.003). Kaplan–Meier analysis demonstrated disease free survival of patients with positive CEA mRNA in post-operative blood to be significantly shorter than in cases negative for CEA mRNA (P=0.03). These results suggest that tumor cells could be shed into the bloodstream during surgical procedures, and these free tumor cells are accompanied by a poor patient outcome. Real-time quantitative RT-PCR is a useful technique for quantitative assessment of free tumor cells in the peripheral blood of colorectal cancer patients.
Keywords :
colorectal neoplasms , Neoplastic circulating cells , Real-time reverse transcriptase-polymerase chain reaction
