Author/Authors :
Ito، نويسنده , , Yasuhiro and Miyoshi، نويسنده , , Eiji and Uda، نويسنده , , Erika and Yoshida، نويسنده , , Hiroshi and Uruno، نويسنده , , Takashi and Takamura، نويسنده , , Yuuki and Miya، نويسنده , , Akihiro and Kobayashi، نويسنده , , Kaoru and Matsuzuka، نويسنده , , Fumio and Matsuura، نويسنده , , Nariaki and Kakudo، نويسنده , , Kennichi and Kuma، نويسنده , , Kanji and Miyauchi، نويسنده , , Akira، نويسنده ,
Abstract :
14-3-3 σ is a negative regulator of the cell cycle and contributes to G2 arrest. Thus far, the lack of its expression due to hypermethylation of the CpG islands has been reported in some carcinomas. In this study, we investigated the expression of 14-3-3 σ in thyroid neoplasms by means of immunohistochemistry as well as Western blot analysis. Normal follicules did not express 14-3-3 σ. In 82 papillary carcinomas, all the cases expressed 14-3-3 σ and its expression was not reduced but even enhanced in the advanced stage and in poorly differentiated types. Furthermore, 21 of the 23 anaplastic carcinomas expressed 14-3-3 σ and its expression level tended to be higher than in papillary carcinoma. On the other hand, none of the 34 follicular carcinomas or 29 follicular adenomas expressed 14-3-3 σ. These results suggest that 14-3-3 σ plays a constitutive role in papillary carcinoma rather than acting as a cell cycle regulator, whereas it is not required for the occurrence and development of follicular tumor.
