Title of article :
Methylation of ASC/TMS1, a proapoptotic gene responsible for activating procaspase-1, in human colorectal cancer
Author/Authors :
Yokoyama، نويسنده , , Taro and Sagara، نويسنده , , Junji and Guan، نويسنده , , Xin and Masumoto، نويسنده , , Junya and Takeoka، نويسنده , , Michiko and Komiyama، نويسنده , , Yuichi and Miyata، نويسنده , , Kazunobu and Higuchi، نويسنده , , Kayoko and Taniguchi، نويسنده , , Shunʹichiro، نويسنده ,
Abstract :
ASC/TMS1, a proapoptotic activator of procaspase-1, was reported to be aberrantly methylated in human breast cancer. We found that ASC was methylated in three of five human colon cancer cell lines lacking ASC protein expression. Demethylation treatment of these cell lines lacking ASC with 5-aza-2′-deoxycytidine partially restored ASC expression. Methylated ASC was also detected in six of ten colorectal cancer tissues. Although clear down-regulation of ASC in the whole region of a tumor tissue was hardly observed by immunostaining with anti-ASC mAb, complete suppression of ASC was identified in a minor population of the colorectal tumor cells. The biological significance of ASC methylation inducible ASC suppression in colorectal cancer will be discussed.
Keywords :
ASC , Colorectal Cancer , Methylation , Down-Regulation
