Author/Authors :
Ron Schondorf، نويسنده , , Thomas and Ebert، نويسنده , , Matthias P. and Hoffmann، نويسنده , , Juliane and Becker، نويسنده , , Martina and Moser، نويسنده , , Natasha and Pur، نويسنده , , ?aziye and G?hring، نويسنده , , Uwe-Jochen and Weisshaar، نويسنده , , Maria-Paz، نويسنده ,
Abstract :
This study was performed to investigate the hypermethylation status of the PTEN gene in ovarian cancer. To this end, we incubated eight ovarian cancer cell lines with the demethylating agent 5-aza-2′ deoxycytidine in three different concentrations for 5 days. Subsequently, the PTEN expression was quantified by both real time RT-PCR and quantitative western analyses. PTEN mRNA varied considerably in response to demethylation whereas PTEN protein concentrations remained constant in all cell lines except OAW42 cells (12.5%). The data suggest that PTEN is highly regulated at translational level. However, methylation of the PTEN gene plays a subordinate role in ovarian cancer.
