Author/Authors :
Sun، نويسنده , , Wenyue and Zhang، نويسنده , , Kaitai and Zhang، نويسنده , , Xinyu and Lei، نويسنده , , Wendong and Xiao، نويسنده , , Ting and Ma، نويسنده , , Jinfang and Guo، نويسنده , , Suping and Shao، نويسنده , , Shujuan and Zhang، نويسنده , , Husheng and Liu، نويسنده , , Yan and Yuan، نويسنده , , Jinsong and Hu، نويسنده , , Zhi and Ma، نويسنده , , Ying and Feng، نويسنده , , Xiaoli and Hu، نويسنده , , Songnian and Zhou، نويسنده , , Jun and Cheng، نويسنده , , Shujun and Gao، نويسنده , , Yanning، نويسنده ,
Abstract :
Lung cancer is one of the major causes of cancer-related deaths. Over the past decade, much has been known about the molecular changes associated with lung carcinogenesis; however, our understanding to lung tumorigenesis is still incomplete. To identify genes that are differentially expressed in squamous cell carcinoma (SCC) of the lung, we compared the expression profiles between primarily cultured SCC tumor cells and bronchial epithelial cells derived from morphologically normal bronchial epithelium of the same patient. Using suppression subtractive hybridization (SSH), two cDNA libraries containing up- and down-regulated genes in the tumor cells were constructed, named as LCTP and LCBP. The two libraries comprise 258 known genes and 133 unknown genes in total. The known up-regulated genes in the library LCTP represented a variety of functional groups; including metabolism-, cell adhesion and migration-, signal transduction-, and anti-apoptosis-related genes. Using semi-quantitative reverse transcription-polymerase chain reaction, seven genes chosen randomly from the LCTP were analyzed in the tumor tissue paired with its corresponding adjacent normal lung tissue derived from 16 cases of the SCC. Among them, the IQGAP1, RAP1GDS1, PAICS, MLF1, and MARK1 genes showed a consistent expression pattern with that of the SSH analysis. Identification and further characterization of these genes may allow a better understanding of lung carcinogenesis.
